Bacteria regulate collective behaviour by mechanosensing cell–cell collisions
Bacterial strainsAll strains and plasmids used in this study are listed in Supplementary Table 1. Gene deletions were constructed by two-step allelic exchange54 following published protocols15. Plasmids were constructed using standard Gibson assembly55 and introduced into P. aeruginosa cells by electroporation. Bacteria were then grown in SOC medium (Corning) for 1 h at 37 °C at 210 … Read more