Peters, AJ, Chen, SX and Komiyama, T. Emergence of reproducible spatiotemporal activity during motor learning. Nature 510, 263–267 (2014). Article ADS CAS PubMed Google Scholar Guo, L. et al. Dynamic rewiring of neuronal circuits in the motor cortex in mouse models of Parkinson’s disease. Nat. Neurosciences. 18, 1299–1309 (2015). CAS Article PubMed PubMed Central Google Scholar Kawai, R. et al. The motor cortex is necessary for learning, but not for executing a motor skill. Neuron 86, 800–812 (2015).CAS Article PubMed PubMed Central Google Scholar Guo, J.-Z. et al. Cortex orders the performance of skillful movements. eLife 4, e10774 (2015). Article PubMed PubMed Central Google Scholar Hwang, EJ et al. Disconnection of the motor cortex from movement control during long-term learning. Science. Adv. 5, eaay0001 (2019).Article ADS CAS PubMed PubMed Central Google Scholar Makino, H., Hwang, EJ, Hedrick, NG & Komiyama, T. Circuit mechanisms of sensorimotor learning. Neuron 92, 705–721 (2016).CAS Article PubMed PubMed Central Google Scholar Peters, AJ, Liu, H. & Komiyama, T. Learning in the rodent motor cortex. Ana. Rev. Neurosciences. 40, 77–97 (2017).CAS Article PubMed PubMed Central Google Scholar Kogan, E., Lu, J. & Zuo, Y. Cortical circuit dynamics underlying motor skill learning: from rodents to humans. Forehead. Mol. Neurosciences. 16, 1292685 (2023).Article PubMed PubMed Central Google Scholar Chen, SX, Kim, AN, Peters, AJ & Komiyama, T. Plasticity of specific subtypes of inhibitory circuits in the motor cortex during motor learning. Nat. Neurosciences. 18, 1109–1115 (2015). CAS Article PubMed PubMed Central Google Scholar Hedrick, NG et al. Learning binds new inputs into functional synaptic clusters through spinogenesis. Nat. Neurosciences. 25, 726–737 (2022).CAS Article PubMed PubMed Central Google Scholar Hedrick, NG, Wright, WJ & Komiyama, T. Local and global predictors of synapse elimination during motor learning. Science. Adv. 10, eadk0540 (2024). CAS Article PubMed PubMed Central Google Scholar Ren, C. et al. Global and subtype-specific modulation of acetylcholine-regulated cortical inhibitory neurons during motor learning. Neuron 110, 2334–2350.e8 (2022).CAS Article PubMed PubMed Central Google Scholar Makino, H. et al. Transformation of emergent properties of the entire cortex during motor learning. Neuron 94, 880–890.e8 (2017).CAS Article PubMed PubMed Central Google Scholar Hwang, EJ, Dahlen, JE, Mukundan, M. & Komiyama, T. Motor cortex disconnection during long-term learning tracks the level of performance of learned movements. J. Neurosci. 41, 7029–7047 (2021). CAS Article PubMed PubMed Central Google Scholar Marshel, JH et al. Specific critical dynamics of the cortical layer that triggers perception. Science 365, eaaw5202 (2019).CAS Article PubMed PubMed Central Google Scholar Packer, AM, Russell, LE, Dalgleish, HWP & Häusser, M. Simultaneous all-optical manipulation and recording of neuronal circuit activity with cellular resolution in vivo. Nat. Methods 12, 140–146 (2015).CAS Article PubMed Google Scholar Adesnik, H. & Abdeladim, L. Probing neural codes with two-photon holographic optogenetics. Nat. Neurosciences. 24, 1356–1366 (2021). CAS Article PubMed PubMed Central Google Scholar Russell, LE et al. All-optical interrogation of neural circuits in behaving mice. Nat. Protocol. 17, 1579–1620 https://doi.org/10.1038/s41596-022-00691-w (2022). CAS Article PubMed PubMed Central Google Scholar Piantadosi Sean, C. et al. Holographic stimulation of opposing amygdala sets bidirectionally modulates valence-specific behavior through mutual inhibition. Neuron 112, 593–610.e5 https://doi.org/10.1016/j.neuron.2023.11.007 (2024).CAS Article PubMed Google Scholar Douglas, RJ & Martin, KAC Neural circuits of the neocortex. Year. Rev. Neurosciences. 27, 419–451 (2004). CAS Article PubMed Google Scholar Douglas, RJ & Martin, KAC Recurrent neural circuits in the neocortex. current. Biol. 17, R496–R500 (2007).CAS Article PubMed Google Scholar Harris, KD & Shepherd, GMG The neocortical circuit: themes and variations. Nat. Neurosciences. 18, 170–181 (2015).CAS Article PubMed PubMed Central Google Scholar Muñoz-Castañeda, R. et al. Cellular anatomy of the mouse primary motor cortex. Nature 598, 159–166 (2021). Article ADS PubMed PubMed Central Google Scholar Oldenburg, IA et al. The logic of recurrent circuits in the primary visual cortex. Nat. Neurosciences. 27, 137–147 (2024).CAS Article PubMed PubMed Central Google Scholar Riehle, A. & Requin, J. The predictive value of performance speed of preparatory changes in neuronal activity of the monkey premotor and motor cortex. Behavior. Brain Res. 53, 35–49 (1993). CAS Article PubMed Google Scholar Churchland, MM, Afshar, A. & Shenoy, KV A central source of movement variability. Neuron 52, 1085–1096 (2006).CAS Article PubMed PubMed Central Google Scholar Churchland, MM, Cunningham, JP, Kaufman, MT, Ryu, SI & Shenoy, KV Cortical preparatory activity: motion representation or first gear in a dynamic machine? Neuron 68, 387–400 (2010).CAS Article PubMed PubMed Central Google Scholar Shenoy, KV, Sahani, M. & Churchland, MM Cortical control of arm movements: a dynamical systems perspective. Year. Rev. Neurosciences. 36, 337–359 (2013). CAS Article PubMed Google Scholar Afshar, A. et al. Single-trial neural correlates of arm movement preparation. Neuron 71, 555–564 (2011).CAS Article PubMed PubMed Central Google Scholar Churchland, MM & Shenoy, KV Preparatory activity and the expansive null space. Nat. Rev. Neurosciences. 25, 213–236 (2024).CAS Article PubMed PubMed Central Google Scholar Vyas, S., Golub, MD, Sussillo, D. & Shenoy, KV Computation through neural population dynamics. Year. Rev. Neurosciences. 43, 249–275 (2020). CAS Article PubMed PubMed Central Google Scholar Sauerbrei, BA et al. Cortical pattern generation during dexterous movement is input-driven. Nature 577, 386–391 (2020).Article ADS CAS PubMed Google Scholar Yuste, R., MacLean, JN, Smith, J. & Lansner, A. The cortex as a central pattern generator. Nat. Rev. Neurosciences. 6, 477–483 (2005). CAS Article PubMed Google Scholar Pandarinath, C. et al. Latent factors and dynamics in the motor cortex and their application to brain-machine interfaces. J. Neurosci. 38, 9390–9401 (2018).CAS Article PubMed PubMed Central Google Scholar Long, MA, Jin, DZ & Fee, MS Support for a synaptic chain model of neuronal sequence generation. Nature 468, 394–399 (2010). Article ADS CAS PubMed PubMed Central Google Scholar Moll, FW et al. The thalamus drives vocal appearances in the zebra finch courtship song. Nature 616, 132–136 (2023). Article ADS CAS PubMed PubMed Central Google Scholar Ramot, A. et al. Motor learning refines thalamic influence on the motor cortex. Nature 643, 725–734 https://doi.org/10.1038/s41586-025-08962-8 (2025). Article ADS CAS PubMed Google Scholar Tanaka, YH et al. Thalamocortical axonal activity in the motor cortex exhibits layer-specific dynamics during motor learning. Neuron 100, 244–258.e12 (2018).CAS Article PubMed Google Scholar Dacre, J. et al. A cerebellar-thalamocortical pathway drives behavioral context-dependent movement initiation. Neuron 109, 2326–2338.e8 (2021). CAS Article PubMed PubMed Central Google Scholar Inagaki, HK et al. A midbrain-thalamus-cortex circuit reorganizes cortical dynamics to initiate movement. Cell 185, 1065–1081.e23 (2022). CAS Article PubMed PubMed Central Google Scholar Takahashi, N. et al. Thalamic input to the motor cortex facilitates the initiation of goal-directed actions. current. Biol. 31, 4148–4155.e4 (2021).CAS Article PubMed PubMed Central Google Scholar Laje, R. & Buonomano, DV Robust synchronization and motor patterns by mastering chaos in recurrent neural networks. Nat. Neurosciences. 16, 925–933 (2013).CAS Article PubMed PubMed Central Google Scholar Bachschmid-Romano, L., Hatsopoulos, NG & Brunel, N. Interaction between external inputs and recurrent dynamics during movement preparation and execution in a motor cortex network model. eLife 12, e77690 (2023).CAS Article PubMed PubMed Central Google Scholar Hennequin, G., Vogels, TP & Gerstner, W. Optimal control of transient dynamics in balanced networks supports the generation of complex motions. Neuron 82, 1394–1406 (2014).CAS Article PubMed PubMed Central Google Scholar Gillett, M., Pereira, U. & Brunel, N. Characteristics of sequential activity in networks with temporally asymmetric Hebbian learning. Proc. Academic National. Science. USA 117, 29948–29958 (2020).Article ADS CAS PubMed PubMed Central Google Scholar Weiler, N., Wood, L., Yu, J., Solla, SA & Shepherd, GMG Top-down laminar organization of the excitatory network in the motor cortex. Nat. Neurosciences. 11, 360–366 (2008). CAS Article PubMed PubMed Central Google Scholar Pachitariu, M. et al. Suite2p: more than 10,000 neurons with standard two-photon microscopy. Preprint on bioRxiv https://doi.org/10.1101/061507 (2017).Chen, Q. et al. Targeted stimulation of motor cortex ensembles drives learned movements. Zenodo https://doi.org/10.5281/zenodo.22216381 (2026).
